概述

  • 产品名称Anti-CYP2A13抗体
    参阅全部 CYP2A13 一抗
  • 描述
    兔多克隆抗体to CYP2A13
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Pig
  • 免疫原

    Synthetic peptide: SVLRDPRFFS NPRDFNPQHF LDKKGQFKKS DAFVPFSIGK RYCFGEGLAR, corresponding to C terminal amino acids 397-446 of Human CYP2A13

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

  • 功能Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
  • 组织特异性Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle.
  • 序列相似性Belongs to the cytochrome P450 family.
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CP2AD_HUMAN antibody
    • CPAD antibody
    • CYP2A antibody
    • CYP2A13 antibody
    • CYPIIA13 antibody
    • Cytochrome P450 2A13 antibody
    • CYTOCHROME P450, SUBFAMILY IIA, POLYPEPTIDE 13 antibody
    see all

Anti-CYP2A13 antibody 图像

  • Anti-CYP2A13 antibody (ab58740) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

    Predicted band size : 57 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

Anti-CYP2A13 antibody (ab58740)参考文献

ab58740 has not yet been referenced specifically in any publications.

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