• 产品名称
    参阅全部 CYP27B1 一抗
  • 描述
    兔多克隆抗体to CYP27B1
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human CYP27B1 aa 482-508 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 1594

  • 阳性对照
    • WB: Mouse kidney tissue lysate. IHC-P: Human kidney tissue. Flow Cyt: 293 cells.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 纯化说明
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • 克隆
  • 同种型


Our Abpromise guarantee covers the use of ab95047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • 功能
    Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
  • 组织特异性
  • 通路
    Hormone biosynthesis; cholecalciferol biosynthesis.
  • 疾病相关
    Defects in CYP27B1 are the cause of rickets vitamin D-dependent type 1A (VDDR1A) [MIM:264700]; also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1alpha(OH)ase antibody
    • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase antibody
    • 25-hydroxyvitamin D-1 alpha hydroxylase antibody
    • 25-OHD-1 alpha-hydroxylase antibody
    • Calcidiol 1-monooxygenase antibody
    • CP27B_HUMAN antibody
    • CP2B antibody
    • CYP1 antibody
    • CYP1ALPHA antibody
    • CYP27B antibody
    • Cyp27b1 antibody
    • Cytochrome p450 27B1 antibody
    • Cytochrome p450 27B13 antibody
    • Cytochrome P450 family 27 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB polypeptide 1 antibody
    • Cytochrome P450C1 alpha antibody
    • Cytochrome P450VD1-alpha antibody
    • mitochondrial antibody
    • P450C1 alpha antibody
    • P450c1 antibody
    • P450C1-alpha antibody
    • P450VD1-alpha antibody
    • PDDR antibody
    • VD3 1A hydroxylase antibody
    • VDD1 antibody
    • VDDR antibody
    • VDDR I antibody
    • VDDRI antibody
    • VDR antibody
    see all

Anti-CYP27B1 antibody 图像

  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + T47D cell line lysates at 20 µg

    Goat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

    Predicted band size : 57 kDa
  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 57 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CYP27B1 with ab95047. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Flow Cytometry analysis of 293 cells labelling CYP27B1 with ab95047 (green) compared to a negative control (blue). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the primary antibody (1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody, a FITC conjugated goat anti-rabbit IgG, was used at 1/200 dilution for 40 min at room temperature. Acquisition of >10,000 events was performed.

Anti-CYP27B1 antibody (ab95047)参考文献

ab95047 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

Read More