The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Recommended: steamed antigen retrieval with citrate
buffer pH 6.
Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.
Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.
ab107527, at a concentration of 3.8 µg/ml, staining CYP26A1 in apparent adhesion sites between axons of paraffin embedded Human cerebellum by Immunohistochemistry. Steamed antigen retrieval with citrate buffer pH 6 and AP-staining were used.