概述

  • 产品名称Anti-CYP21A2抗体
    参阅全部 CYP21A2 一抗
  • 描述
    兔多克隆抗体to CYP21A2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human CYP21A2.

  • 阳性对照
    • extracts from 293 cells.

应用

Our Abpromise guarantee covers the use of ab64887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/20,000.
    WB: 1/500 - 1/100. Detects bands of approximately 56 and 40 kDa (predicted molecular weight: 56 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
    • 疾病相关Adrenal hyperplasia 3
    • 序列相似性Belongs to the cytochrome P450 family.
    • 结构域The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
    • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CYP21 antibody
      • 21 OHase antibody
      • 21-OHase antibody
      • CA21H antibody
      • CAH1 antibody
      • CP21A_HUMAN antibody
      • CPS1 antibody
      • CYP21A 2 antibody
      • CYP21A2 antibody
      • CYP21B antibody
      • Cytochrome P-450c21 antibody
      • Cytochrome P450 21 antibody
      • Cytochrome P450 C21B antibody
      • Cytochrome P450 XXI antibody
      • Cytochrome P450, family 21, subfamily A, polypeptide 2 antibody
      • Cytochrome P450, subfamily XXIA (steroid 21 hydroxylase, congenital adrenal hyperplasia), polypeptide 2 antibody
      • Cytochrome P450-C21 antibody
      • Cytochrome P450-C21B antibody
      • P450 C21 antibody
      • P450 C21B antibody
      • P450c21B antibody
      • Steroid 21 hydroxylase antibody
      • Steroid 21 monooxygenase antibody
      • Steroid 21-hydroxylase antibody
      see all

    Anti-CYP21A2 antibody 图像

    • All lanes : Anti-CYP21A2 antibody (ab64887) at 1/500 dilution

      Lane 1 : extracts from 293 cells (5-30µg total protein)
      Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


      Predicted band size : 56 kDa
      Observed band size : 56 kDa
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Anti-CYP21A2 antibody (ab64887)参考文献

    This product has been referenced in:
    • Bouhaddioui W  et al. CYP21A2 expression is localized in the developing distal epithelium of the human perinatal lung and is compatible with in situ production and intracrine actions of active glucocorticoids. J Steroid Biochem Mol Biol 163:12-9 (2016). Read more (PubMed: 27004467) »
    • Idkowiak J  et al. Concomitant mutations in the P450 oxidoreductase and androgen receptor genes presenting with 46,XY disordered sex development and androgenization at adrenarche. J Clin Endocrinol Metab 95:3418-27 (2010). WB ; Human . Read more (PubMed: 20410220) »

    See all 2 Publications for this product

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    Thank you for contacting us. The immunogen sequence is from 150aa to 200aa. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"