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Our Abpromise guarantee covers the use of ab78044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24497629|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 61 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
Immunocytochemistry/Immunofluorescence analysis of COS-7 cells (grown on coverslips were transfected with vector alone or wild wtype (WT) CYP1B1 cDNAs) labelling CYP1B1 with ab78044 at 1/100. The cells were fixed with chilled methanol, and cells were also immunostained with a mouse anti-CcOI (1/100). The cells were then stained with Alexa 488-conjugated anti-rabbit antibody and Alexa 594-conjugated anti-mouse IgG (both 1/100). The stained patterns were overlaid.
Image courtesy of an anonymous Abreview.
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