概述

  • 产品名称
  • 描述
    兔多克隆抗体to CYP1B1
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human CYP1B1.

    (Peptide available as ab33585.)

  • 阳性对照
    • Human brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab33586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).Can be blocked with Human CYP1B1 peptide (ab33585).
ICC 1/1000.
ICC/IF 1/500. (see Abreview)

靶标

  • 功能
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
  • 组织特异性
    Expressed in many tissues.
  • 疾病相关
    Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
    Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
    Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aryl hydrocarbon hydroxylase antibody
    • CP1B antibody
    • CP1B1_HUMAN antibody
    • Cyp1b1 antibody
    • CYPIB1 antibody
    • Cytochrome P450 1B1 antibody
    • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
    • Flavoprotein linked monooxygenase antibody
    • GLC3A antibody
    • Microsomal monooxygenase antibody
    • P4501B1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Anti-CYP1B1 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-CYP1B1 antibody (ab33586) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Human brain tissue lysate at 10 µg

    Secondary
    Lane 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : ~70 kDa (why is the actual band size different from the predicted?)
  • ab33586 staining CYP1B1 in Human AGS Human gastric carcinoma cultured cells by Immunocytochemistry (ICC). Tissue was fixed with formaldehyde, permeabilized with 0.025% Triton-X in PBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-CYP1B1 antibody (ab33586)参考文献

This product has been referenced in:

See all 5 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (AGS Human gastric carcinoma cells)
Specification
AGS Human gastric carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

提交于 Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Human gastric carcinoma cells)
Specification
AGS Human gastric carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS human gastric carcinoma cells)
Loading amount
20 µg
Specification
AGS human gastric carcinoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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