Anti-CYP11A1抗体(ab86357)

概述

  • 产品名称
    Anti-CYP11A1抗体
    参阅全部 CYP11A1 一抗
  • 描述
    兔多克隆抗体to CYP11A1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human CYP11A1.

    (Peptide available as ab95404.)

  • 阳性对照
    • This antibody gave a positive signal in the following human lysates: HeLa Whole Cell; HepG2 Whole Cell; Brain Tissue; Testis Tissue; Liver Tissue.

应用

Our Abpromise guarantee covers the use of ab86357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能
    Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
  • 通路
    Lipid metabolism; C21-steroid hormone metabolism.
  • 疾病相关
    Defects in CYP11A1 are a cause of congenital adrenal insufficiency (CAI).
    Defects in CYP11A1 are a cause of congenital lipoid adrenal hyperplasia (CLAH) [MIM:201710]; also known as lipoid CAH. CLAH is the most severe form of adrenal hyperplasia. This autosomal recessive and potentially lethal condition includes the onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. CLAH is a rare disease, except in Japan and Korea where it accounts for a significant percentage of cases of congenital adrenal hyperplasia.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Cholesterol 20 22 desmolase antibody
      • Cholesterol desmolase antibody
      • Cholesterol monooxygenase (side chain cleaving) antibody
      • Cholesterol side chain cleavage enzyme antibody
      • Cholesterol side chain cleavage enzyme mitochondrial antibody
      • Cholesterol side-chain cleavage enzyme antibody
      • CP11A_HUMAN antibody
      • CYP11A antibody
      • CYP11A1 antibody
      • CYPXIA1 antibody
      • Cytochrome P450 11A1 antibody
      • Cytochrome P450 11A1 mitochondrial antibody
      • Cytochrome P450 family 11 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XIA antibody
      • Cytochrome P450(scc) antibody
      • Cytochrome P450C11A1 antibody
      • mitochondrial antibody
      • P450SCC antibody
      • Steroid 20 22 lyase antibody
      see all

    Anti-CYP11A1 antibody 图像

    • All lanes : Anti-CYP11A1 antibody (ab86357) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human brain tissue lysate - total protein (ab29466)
      Lane 3 : Human testis tissue lysate - total protein (ab30257)
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP))
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
      Additional bands at : 55 kDa (possible cleavage fragment),80 kDa,85 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 15 minutes

    实验方案

    Anti-CYP11A1 antibody (ab86357)参考文献

    ab86357 has not yet been referenced specifically in any publications.

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