概述

  • 产品名称Anti-CYLC2抗体
    参阅全部 CYLC2 一抗
  • 描述
    兔多克隆抗体to CYLC2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 300-349 (DAKKVAKKDT EKESADSKKD AKKNAKKDAK KDAKKNAKKD EKKDAKKKGK) of Human CYLC2 (NP_001331).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 相关性CYCL2 (Cylicin II) is specifically expressed in testis and is part of the cytoskeletal calyx of mammalian sperm heads. Cylicin II may play a role in the morphogenesis of the sperm head and may be involved in spermatid differentiation.
  • 细胞定位Cytoplasm, cytoskeleton, perinuclear theca, calyx. Note=Sperm head cytoskeletal structure.
  • 数据库链接
  • 别名
    • CYL2 antibody
    • CYLC 2 antibody
    • Cylicin 2 antibody
    • Cylicin basic protein of sperm head cytoskeleton 2 antibody
    • Cylicin II antibody
    • Cylicin2 antibody
    • MGC129591 antibody
    • Multiple band polypeptide II antibody
    see all

Anti-CYLC2 antibody 图像

  • Anti-CYLC2 antibody (ab81228) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-CYLC2 antibody (ab81228)参考文献

ab81228 has not yet been referenced specifically in any publications.

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