CyGEL Sustain™ (ab109205)
概述
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产品名称
CyGEL Sustain™ -
经测试应用
适用于: ICC/IF, FMmore details -
常规说明
CyGEL Sustain™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL Sustain™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling. CyGEL Sustain™ has many applications in imaging and cell-based screening.
Key features of CyGEL Sustain™ include:- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick perparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
For live C. elegans and parasites we recommend CyGEL™(ab109204).
性能
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形式
Liquid -
存放说明
Store at +4°C. Do not freeze. -
存储溶液
Constituent: 100% Oxirane methyl polymer with oxirane -
Concentration information loading...
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109205于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain. |
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FM |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use at an assay dependent concentration. Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain. |
FM
Use at an assay dependent concentration. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109205 被引用在 1 文献中.
- Fritzsche M et al. Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation. Sci Adv 3:e1603032 (2017). PubMed: 28691087