概述

  • 产品名称
  • 描述
    兔多克隆抗体to CYFIP1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1203 and 1253 of Human CYFIP1 (NP_055423.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab108220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
  • 序列相似性
    Belongs to the CYFIP family.
  • 细胞定位
    Cytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CY antibody
    • CYFIP 1 antibody
    • CYFIP1 antibody
    • CYFP1_HUMAN antibody
    • Cytoplasmic FMR1 interacting protein 1 antibody
    • Cytoplasmic FMR1-interacting protein 1 antibody
    • FLJ45151 antibody
    • KIAA0068 antibody
    • p140sra 1 antibody
    • p140sra-1 antibody
    • P140SRA1 antibody
    • Selective hybridizing clone antibody
    • SHYC antibody
    • Specifically Rac1 associated protein 1 antibody
    • Specifically Rac1-associated protein 1 antibody
    • SRA 1 antibody
    • Sra-1 antibody
    • SRA1 antibody
    see all

图片

  • All lanes : Anti-CYFIP1 antibody (ab108220) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 145 kDa


    Exposure time : 3 minutes
  • ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of CYFIP1
    Lane 2: ab108220 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds

文献

This product has been referenced in:
  • Han K  et al. Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice. Hum Mol Genet 24:1813-23 (2015). Read more (PubMed: 25432536) »

See 1 Publication for this product

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