Anti-Cyclosporin A抗体[CSZ.22] (ab8312)

Abreviews (1)Q&A (4)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-Cyclosporin A抗体[CSZ.22]
  • 描述
    小鼠单克隆抗体[CSZ.22] to Cyclosporin A
  • 特异性
    This antibody recognises Cyclosporin A in buffer solutions and whole blood extracts with Kd = 10-8
  • 经测试应用
    适用于: ELISAmore details
  • 常规说明


    This antibody recognises Cyclosporin A

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    This antibody recognises Cyclosporin A
  • 克隆
    单克隆
  • 克隆编号
    CSZ.22
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1500.

Use at a dilution of 1/1500 for 2 hrs.

靶标

  • 相关性
    Cyclosporin is used to prevent rejection following solid organ and bone marrow transplantation . It has been estimated that cyclosporine is given to more than 90% of children who have received a kidney transplant in the United States. In addition, the ability of cyclosporin to inhibit T-cell activation has been shown to have a role in the treatment of diseases such as nephrotic syndrome, refractory Crohn's disease and ulcerative colitis, biliary cirrhosis, aplastic anemia, rheumatoid arthritis, myasthenia gravis, and dermatomyositis.
  • 细胞定位
    Cytoplasmic

Anti-Cyclosporin A antibody [CSZ.22] 图像

  • ELISA - Cyclosporin A antibody [CSZ.22] (ab8312)
    ELISA - Cyclosporin A antibody [CSZ.22] (ab8312)This image is courtesy of an anonymous Abreview

    ab8312 staining human PBMC, huh-7 cells by ELISA.  Cells were incubated in their respective growth medium with 10% fetal calf serum. At the end of the incubation they were washed with PBS (4°C) and the pellet was used to determine the CsA content. 

    1st step: cyclophilin B (ab53870, 1/1500 carbonate pH 8.5) was bound to the plate (overnight 4°C), 2nd step blocking agent, 3rd step incubation with cell lysate incubated with CsA or analogs (CsA-OH) overnight,
    4th step ab8312 mouse anti CsA, 1/500 for 2 hours at 37°C
    5th step ab6729 (1/2000) Alkalline phosphatase reading at 405 nm

    See Abreview

Anti-Cyclosporin A antibody [CSZ.22] (ab8312)参考文献

ab8312 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab8312？请让我们知道，以便我们可以引用本数据表中的参考文章。

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5 Abreviews or Q&A

10
Votes: Highest First

Could you send me the part number and the secondary antibody for this particular primary antibody.The samples i will be dealing for detection are obtained from rat.

Thanks!

Thank you very much for your reply.

When using samples like blood that contain endogenous IgG's, I recommend using a pre-adsorbed secondary antibody that won't bind to the IgG molecules in your samples. Using ab8312 as the primary antibody, I...

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I will be using the ELISA assay and would like to give it a try for the synthetic CsA. Could you send me a detail protocol and also the price and catalog number for all the materials required.

Thanks!

Regards,

Thank you for your call today and for your patience while I've been in touch with the production lab regarding this antibody.

As we discussed, ab8312 has only been tested in indirect ELISA by coating the plate with antigen and adding the anti...

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Hi,

I wanted to inquire about this antibody. I wanted to use it to detect cyclosporine (chemically synthesized) in rat tissues and blood.Do you think it could be used for that purpose.Thanks!

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Thank you very much for contacting us with your question.

The cyclosporin A antibody, ab8312, is suitable for detecting synthesized cyclosporin in rat samples, however it has only been tested in ELISA at this time. What kind of assay would yo...

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I would like to test this antibody in immunocytochemistry/immunofluorescence (ICC/IF), an untested application.

Thank you very much for your interest in ab8312.

To our knowledge, ab8312 has not been tested inICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab8312 now, test it in ICC/IFand submit feedback to us in the form of ...

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ELISA abreview for Anti-Cyclosporin A antibody [CSZ.22]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (PBMC, huh-7 cells)
Specification
PBMC, huh-7 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type
Sandwich (Detection)
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提交于 Jun 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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