概述
- 产品名称Anti-Cyclosporin A抗体[CSZ.22]
- 描述小鼠单克隆抗体[CSZ.22] to Cyclosporin A
- 特异性This antibody recognises Cyclosporin A in buffer solutions and whole blood extracts with Kd = 10-8
- 经测试应用适用于: ELISAmore details
- 常规说明
This antibody recognises Cyclosporin A
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液PBS with 0.1% sodium azide, pH 7.4
-
Concentration information loading... - 纯度Protein G purified
- Primary antibody说明This antibody recognises Cyclosporin A
- 克隆单克隆
- 克隆编号CSZ.22
- 同种型IgG1
- 轻链类型unknown
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab8312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/1500. Use at a dilution of 1/1500 for 2 hrs. |
靶标
- 相关性Cyclosporin is used to prevent rejection following solid organ and bone marrow transplantation . It has been estimated that cyclosporine is given to more than 90% of children who have received a kidney transplant in the United States. In addition, the ability of cyclosporin to inhibit T-cell activation has been shown to have a role in the treatment of diseases such as nephrotic syndrome, refractory Crohn's disease and ulcerative colitis, biliary cirrhosis, aplastic anemia, rheumatoid arthritis, myasthenia gravis, and dermatomyositis.
- 细胞定位Cytoplasmic
Anti-Cyclosporin A antibody [CSZ.22] 图像
-
![ELISA - Cyclosporin A antibody [CSZ.22] (ab8312)](http://www.abcam.cn/ps/datasheet/Images/8/ab8312/ab8312_1.jpg)
ELISA - Cyclosporin A antibody [CSZ.22] (ab8312)This image is courtesy of an anonymous Abreviewab8312 staining human PBMC, huh-7 cells by ELISA. Cells were incubated in their respective growth medium with 10% fetal calf serum. At the end of the incubation they were washed with PBS (4°C) and the pellet was used to determine the CsA content.
1st step: cyclophilin B (ab53870, 1/1500 carbonate pH 8.5) was bound to the plate (overnight 4°C), 2nd step blocking agent, 3rd step incubation with cell lysate incubated with CsA or analogs (CsA-OH) overnight,
4th step ab8312 mouse anti CsA, 1/500 for 2 hours at 37°C
5th step ab6729 (1/2000) Alkalline phosphatase reading at 405 nm
Anti-Cyclosporin A antibody [CSZ.22] (ab8312)参考文献
ab8312 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"