概述
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产品名称Anti-Cyclosporin A抗体[CSZ.22]
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描述小鼠单克隆抗体[CSZ.22] to Cyclosporin A
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宿主Mouse
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特异性This antibody recognises Cyclosporin A in buffer solutions and whole blood extracts with Kd = 10-8
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经测试应用适用于: ELISAmore details
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常规说明
This antibody recognises Cyclosporin A
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液PBS with 0.1% sodium azide, pH 7.4
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Concentration information loading... -
纯度Protein G purified
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Primary antibody说明This antibody recognises Cyclosporin A
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克隆单克隆
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克隆编号CSZ.22
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同种型IgG1
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab8312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/1500. Use at a dilution of 1/1500 for 2 hrs. |
靶标
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相关性Cyclosporin is used to prevent rejection following solid organ and bone marrow transplantation . It has been estimated that cyclosporine is given to more than 90% of children who have received a kidney transplant in the United States. In addition, the ability of cyclosporin to inhibit T-cell activation has been shown to have a role in the treatment of diseases such as nephrotic syndrome, refractory Crohn's disease and ulcerative colitis, biliary cirrhosis, aplastic anemia, rheumatoid arthritis, myasthenia gravis, and dermatomyositis.
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细胞定位Cytoplasmic
图片
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![ELISA - Anti-Cyclosporin A antibody [CSZ.22] (ab8312)](http://www.abcam.cn/ps/products/8/ab8312/Images/ab8312_1.jpg)
ELISA - Anti-Cyclosporin A antibody [CSZ.22] (ab8312)This image is courtesy of an anonymous Abreviewab8312 staining human PBMC, huh-7 cells by ELISA. Cells were incubated in their respective growth medium with 10% fetal calf serum. At the end of the incubation they were washed with PBS (4°C) and the pellet was used to determine the CsA content.
1st step: cyclophilin B (ab53870, 1/1500 carbonate pH 8.5) was bound to the plate (overnight 4°C), 2nd step blocking agent, 3rd step incubation with cell lysate incubated with CsA or analogs (CsA-OH) overnight,
4th step ab8312 mouse anti CsA, 1/500 for 2 hours at 37°C
5th step ab6729 (1/2000) Alkalline phosphatase reading at 405 nm
文献
ab8312 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"