Alexa Fluor® 488荧光Anti-Cyclophilin F抗体[E11AE12BD4] (ab156032)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-Cyclophilin F抗体[E11AE12BD4]
参阅全部 Cyclophilin F 一抗 -
描述
Alexa Fluor® 488荧光小鼠单克隆抗体[E11AE12BD4] to Cyclophilin F -
宿主
Mouse -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant full length protein corresponding to Rat Cyclophilin F.
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阳性对照
- HDFn and HeLa cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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纯化说明
ab156032 is a conjugated form of ab110324 which was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
E11AE12BD4 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use a concentration of 0.2 µg/ml.
ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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Flow Cyt (Intra)
Use a concentration of 0.2 µg/ml. ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
序列相似性
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 414346 Cow
- Entrez Gene: 10105 Human
- Entrez Gene: 105675 Mouse
- Entrez Gene: 282819 Rat
- Omim: 604486 Human
- SwissProt: P30404 Cow
- SwissProt: P30405 Human
- SwissProt: Q99KR7 Mouse
see all -
形式
This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein. -
别名
- Cyclophilin 3 antibody
- cyclophilin D antibody
- Cyclophilin F antibody
see all
图片
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml, (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum. -
Immunofluorescent analysis of paraformaldehyde-fixed HDFn cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum -
Flow cytometric analysis of HeLa cells labeling Cyclophilin F with ab156032 at 0.2 µg/ml (red) or an equal amount of IgG1 Alexa-488 Isotype control antibody (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab156032 尚未被引用在任何文献中。