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Anti-Cyclophilin F抗体[E11AE12BD4] (ab110324)

概述

  • 产品名称
    Anti-Cyclophilin F抗体[E11AE12BD4]
    参阅全部 Cyclophilin F 一抗
  • 描述
    小鼠单克隆抗体[E11AE12BD4] to Cyclophilin F
  • 经测试应用
    适用于: WB, Flow Cyt, In-Cell ELISA, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein corresponding to Rat Cyclophilin F aa 1-206. (also known as CypD)

  • 阳性对照
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, HepG2 cells; Cultured Human embryonic lung-derived fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.
  • 常规说明

    This monoclonal antibody to cyclophilin F has been knockout validated in Western blot. The expected band for cyclophilin F was observed in wild type cells and the band was not seen in knockout cells.

性能

应用

Our Abpromise guarantee covers the use of ab110324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

In-Cell ELISA Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP 1/1000.

靶标

  • 功能
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 序列相似性
    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 形式
    This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • 别名
    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Anti-Cyclophilin F antibody [E11AE12BD4] 图像



  • Predicted band size : 22 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Cyclophilin F knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab110324 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab110324 detected the expected band for Cyclophilin F in wild type HAP1 cells and the band was not seen in Cyclophilin F knockout HAP1 cells. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE.  Ab110324 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry analysis using ab110324 at 1µg/ml staining Cyclophilin 40 in Cultured Human embryonic lung-derived fibroblasts (strain MRC5), (fixed, treated for heat-induced antigen retrieval, permeabilized) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
  • All lanes : Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) at 1/1000 dilution

    Lane 1 : WT mouse liver mitochondria lysate at 25 µg
    Lane 2 : CypD KO mouse liver mitochondria lysate at 25 µg
    Lane 3 : WT mouse liver mitochondria lysate at 35 µg
    Lane 4 : CypD KO mouse liver mitochondria lysate at 35 µg
    Lane 5 : WT mouse liver mitochondria lysate at 50 µg
    Lane 6 : CypD KO mouse liver mitochondria lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa (possible non-specific binding).

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25°C.

    Incubated with the primary antibody diluted in PBS-T + 5% milk for 16 hours at 4°C.

    See Abreview

  • Immunohistological analysis using ab110324 at 1µg/ml staining Cyclophilin F in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
    Note: immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
  • Immunocytochemistry/ Immunofluorescence analysis of HEK293 cells labeling Cyclophilin F with ab110324 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with 1% triton x-100. 10% goat serum was used to blocke the cells for 1 hour at room temp followed by incubation with Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) in 10% goat serum-PBST for 16 hours at 4°C. A goat anti-mouse IgG secondary antibody was used at 1/300 dilution.

    See Abreview

  • All lanes : Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : Isolated mitochondria from HepG2 cells at 20 µg


    Predicted band size : 22 kDa
  • Flow cytometric analysis using ab110324 at 1µg/ml staining Cyclophilin F in HL60 cells (blue). Isotype control antibody (red).

Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)参考文献

This product has been referenced in:
  • Vial G  et al. Liver mitochondrial function in ZDF rats during the early stages of diabetes disease. Physiol Rep 4:N/A (2016). WB ; Rat . Read more (PubMed: 26847727) »
  • Chourasia AH  et al. Mitophagy defects arising from BNip3 loss promote mammary tumor progression to metastasis. EMBO Rep 16:1145-63 (2015). ICC/IF ; Mouse . Read more (PubMed: 26232272) »

See all 35 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO cells)
Permeabilization
Yes - 1% triton x-100
Specification
CHO cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 1% triton x-100
Specification
HEK293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (H9C2)
Permeabilization
Yes - 1% triton x-100
Specification
H9C2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Permeabilization
Yes - 1% triton x-100
Specification
MEFs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 10 2016

Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse liver mitochondria)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
Mouse liver mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 01 2015

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Total protein in input
10 µg
Username

Abcam user community

Verified customer

提交于 Mar 05 2014


Sachez que ces anticorps sont contre la cyclophiline F ', PPIF, CYP3, SwissProt: P30405. Toutefois, dans le domaine mitochondrial, cette protéine est généralement appelé CYPD ou cyclophiline D - une protéine de 25 kDa dans les mitochondries.
...

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Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten, nur weil wir einen Datenblattfehler gemacht haben. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auf...

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The antibody was raised against full length recombinant rat CypD protein.

The identity between cyp A and “cypD” (cylophilin F, PPIF) is only 75% which is not high.

Th...

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