Anti-Cyclophilin F抗体[E11AE12BD4] (ab110324)

概述

  • 产品名称Anti-Cyclophilin F抗体[E11AE12BD4]
    参阅全部 Cyclophilin F 一抗
  • 描述
    小鼠单克隆抗体[E11AE12BD4] to Cyclophilin F
  • 经测试应用适用于: WB, Flow Cyt, In-Cell ELISA, ICC/IF, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein corresponding to Rat Cyclophilin F aa 1-206. (also known as CypD)

  • 阳性对照
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, HepG2 cells; Cultured Human embryonic lung-derived fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.

性能

应用

Our Abpromise guarantee covers the use of ab110324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

In-Cell ELISA Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP 1/1000.

靶标

  • 功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 序列相似性Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 形式This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • 别名
    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Anti-Cyclophilin F antibody [E11AE12BD4] 图像

  • All lanes : Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) at 1/1000 dilution

    Lane 1 : WT mouse liver mitochondria lysate at 25 µg
    Lane 2 : CypD KO mouse liver mitochondria lysate at 25 µg
    Lane 3 : WT mouse liver mitochondria lysate at 35 µg
    Lane 4 : CypD KO mouse liver mitochondria lysate at 35 µg
    Lane 5 : WT mouse liver mitochondria lysate at 50 µg
    Lane 6 : CypD KO mouse liver mitochondria lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa (possible non-specific binding).

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25°C.

    Incubated with the primary antibody diluted in PBS-T + 5% milk for 16 hours at 4°C.

    See Abreview

  • All lanes : Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : Isolated mitochondria from HepG2 cells at 20 µg


    Predicted band size : 22 kDa
  • Immunocytochemistry analysis using ab110324 at 1µg/ml staining Cyclophilin 40 in Cultured Human embryonic lung-derived fibroblasts (strain MRC5), (fixed, treated for heat-induced antigen retrieval, permeabilized) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
  • Flow cytometric analysis using ab110324 at 1µg/ml staining Cyclophilin F in HL60 cells (blue). Isotype control antibody (red).
  • Immunohistological analysis using ab110324 at 1µg/ml staining Cyclophilin F in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
    Note: immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)参考文献

This product has been referenced in:
  • Chourasia AH  et al. Mitophagy defects arising from BNip3 loss promote mammary tumor progression to metastasis. EMBO Rep 16:1145-63 (2015). ICC/IF ; Mouse . Read more (PubMed: 26232272) »
  • Qiu F  et al. Arginine starvation impairs mitochondrial respiratory function in ASS1-deficient breast cancer cells. Sci Signal 7:ra31 (2014). Human . Read more (PubMed: 24692592) »

See all 35 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO cells)
Permeabilization Yes - 1% triton x-100
Specification CHO cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293)
Permeabilization Yes - 1% triton x-100
Specification HEK293
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (H9C2)
Permeabilization Yes - 1% triton x-100
Specification H9C2
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Aug 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification CHO cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEFs)
Permeabilization Yes - 1% triton x-100
Specification MEFs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Aug 10 2016

Application Western blot
Sample Mouse Tissue lysate - other (Mouse liver mitochondria)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Mouse liver mitochondria
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 01 2015

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Tissue lysate - whole (brain)
Specification brain
Total protein in input 10 µg
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提交于 Mar 05 2014


Sachez que ces anticorps sont contre la cyclophiline F ', PPIF, CYP3, SwissProt: P30405. Toutefois, dans le domaine mitochondrial, cette protéine est généralement appelé CYPD ou cyclophiline D - une protéine de 25 kDa dans les mitochondries.
...

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Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten, nur weil wir einen Datenblattfehler gemacht haben. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auf...

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The antibody was raised against full length recombinant rat CypD protein.

The identity between cyp A and “cypD” (cylophilin F, PPIF) is only 75% which is not high.

Th...

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