Anti-Cyclophilin F抗体[AT1F5] (ab86425)

概述

  • 产品名称Anti-Cyclophilin F抗体[AT1F5]
    参阅全部 Cyclophilin F 一抗
  • 描述
    小鼠单克隆抗体[AT1F5] to Cyclophilin F
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 30-207 of Human Cyclophilin F, purified from E. coli

  • 阳性对照
    • 293T and HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab86425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/50.

靶标

  • 功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 序列相似性Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 形式This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • 别名
    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Anti-Cyclophilin F antibody [AT1F5] 图像

  • All lanes : Anti-Cyclophilin F antibody [AT1F5] (ab86425) at 1/500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 22 kDa
  • ab86425 staining Cyclophilin F in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection. 

Anti-Cyclophilin F antibody [AT1F5] (ab86425)参考文献

ab86425 has not yet been referenced specifically in any publications.

Product Wall


Sachez que ces anticorps sont contre la cyclophiline F ', PPIF, CYP3, SwissProt: P30405. Toutefois, dans le domaine mitochondrial, cette protéine est généralement appelé CYPD ou cyclophiline D - une protéine de 25 kDa dans les mitochondries.
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