Recombinant human Cyclophilin B (26-216 aa) purified from E. coli
ab74173, clone k2E2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclophilin B protein.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab74173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB | 1/1000 - 1/2000. Predicted molecular weight: 23 kDa. | |
ELISA | Use at an assay dependent concentration. | |
ICC/IF | Use a concentration of 1 µg/ml. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"