Anti-Cyclophilin B抗体[k2E2] (ab74173)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-Cyclophilin B抗体[k2E2]
参阅全部 Cyclophilin B 一抗 -
描述小鼠单克隆抗体[k2E2] to Cyclophilin B
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特异性This antibody does not cross react with Cyclophilin A, D, E, F, G and H.
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经测试应用适用于: IHC-P, Flow Cyt, WB, ELISA, ICC/IFmore details
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种属反应性与反应: Mouse, Human
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免疫原
Recombinant human Cyclophilin B (26-216 aa) purified from E. coli
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阳性对照
- HepG2 and HeLa cell lysates.
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常规说明
ab74173, clone k2E2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclophilin B protein.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
纯度Protein G purified
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Primary antibody说明ab74173, clone k2E2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclophilin B protein.
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克隆单克隆
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克隆编号k2E2
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同种型IgG1
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轻链类型kappa
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab74173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 23 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
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疾病相关Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
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序列相似性Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain. -
细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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数据库链接
- Entrez Gene: 5479 Human
- Entrez Gene: 19035 Mouse
- Omim: 123841 Human
- SwissProt: P23284 Human
- SwissProt: P24369 Mouse
- Unigene: 434937 Human
- Unigene: 335249 Mouse
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别名
- AA408962 antibody
- AA553318 antibody
- AI844835 antibody
see all
Anti-Cyclophilin B antibody [k2E2] 图像
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![Western blot - Cyclophilin B antibody [k2E2] (ab74173)](http://www.abcam.cn/ps/datasheet/Images/74/ab74173/ab74173wb.gif)
Western blot - Cyclophilin B antibody [k2E2] (ab74173)All lanes : Anti-Cyclophilin B antibody [k2E2] (ab74173) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
goat anti-mouse antibody conjugated to HRP
Developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?) -
](http://www.abcam.cn/ps/datasheet/images/74/ab74173/Cyclophilin-B-Primary-antibodies-ab74173-1.jpg)
Immunocytochemistry/ Immunofluorescence-Cyclophilin B antibody [k2E2](ab74173)ICC/IF image of ab74173 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74173, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
](http://www.abcam.cn/ps/datasheet/images/74/ab74173/Cyclophilin-B-Primary-antibodies-ab74173-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cyclophilin B antibody [k2E2](ab74173)IHC image of ab74173 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab74173, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-Cyclophilin B antibody [k2E2] (ab74173)](http://www.abcam.cn/ps/datasheet/images/74/ab74173/Cyclophilin-B-Primary-antibodies-ab74173-23.jpg)
Flow Cytometry - Anti-Cyclophilin B antibody [k2E2] (ab74173)Overlay histogram showing HeLa cells stained with ab74173 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab74173, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
Anti-Cyclophilin B antibody [k2E2] (ab74173)参考文献
This product has been referenced in:
- Dubnikov T et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. J Cell Sci 129:3635-3647 (2016). Read more (PubMed: 27550517) »
- Choi JW et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. PLoS Genet 5:e1000750 (2009). WB ; Mouse . Read more (PubMed: 19997487) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"