This antibody gave a positive signal in the following human lysates:
HeLa (Human epithelial carcinoma cell line) whole cell
A431 (Human epithelial carcinoma cell line) whole cell
Lymph node (Human) whole cell - adult normal tissue
This antibody gave a positive signal in the following rat lysate:
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at in the range of 1/100 - 1/500 depending on incubation time/ condtions of primary.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily. Contains 1 PPIase cyclophilin-type domain.
Epididymis secretory sperm binding protein Li 69p antibody
HEL S 69p antibody
Peptidyl prolyl cis trans isomerase A antibody
Peptidyl-prolyl cis-trans isomerase A antibody
Peptidylprolyl isomerase A (cyclophilin A) antibody
Peptidylprolyl isomerase A antibody
PPIA protein antibody
PPIase A antibody
Rotamase A antibody
T cell cyclophilin antibody
Western blot - Cyclophilin A antibody (ab42408)
All lanes : Anti-Cyclophilin A antibody (ab42408) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Human lymph node tissue lysate - total protein (ab29871) Lane 4 : Human thymus tissue lysate - total protein (ab30146) Lane 5 : Lung (Rat) Tissue Lysate
Predicted band size : 18 kDa Observed band size : 18 kDa
Anti-Cyclophilin A antibody (ab42408) at 1/1000 dilution + Recombinant Human Cyclophilin A protein (ab96022) at 0.1 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - Cyclophilin A antibody (ab42408)
ICC/IF image of ab42408 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42408, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclophilin A antibody (ab42408)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab42408 staining Cyclophilin A in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocked (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used, undiluted as secondary.
Lin AL et al. Rapamycin rescues vascular, metabolic and learning deficits in apolipoprotein E4 transgenic mice with pre-symptomatic Alzheimer's disease. J Cereb Blood Flow Metab37:217-226 (2017).
Read more (PubMed: 26721390) »
Heinzmann D et al. The Novel Extracellular Cyclophilin A (CyPA) - Inhibitor MM284 Reduces Myocardial Inflammation and Remodeling in a Mouse Model of Troponin I -Induced Myocarditis. PLoS One10:e0124606 (2015).
Read more (PubMed: 25894208) »