This antibody gave a positive signal in the following human lysates:
HeLa (Human epithelial carcinoma cell line) whole cell
A431 (Human epithelial carcinoma cell line) whole cell
Lymph node (Human) whole cell - adult normal tissue
This antibody gave a positive signal in the following rat lysate:
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Epididymis secretory sperm binding protein Li 69p antibody
HEL S 69p antibody
Peptidyl prolyl cis trans isomerase A antibody
Peptidyl-prolyl cis-trans isomerase A antibody
Peptidylprolyl isomerase A (cyclophilin A) antibody
Peptidylprolyl isomerase A antibody
PPIA protein antibody
PPIase A antibody
Rotamase A antibody
T cell cyclophilin antibody
Western blot - Anti-Cyclophilin A antibody (ab41684)
All lanes : Anti-Cyclophilin A antibody (ab41684) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : Human lymph node tissue lysate - total protein (ab29871) Lane 4 : Human thymus tissue lysate - total protein (ab30146) Lane 5 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa Observed band size: 18 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684)
ICC/IF image of ab41684 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41684, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody (ab41684)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd
ab41684 staining mouse heart and rat thymus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab41684)This image is courtesy of an abreview submitted by Alina Macovei.
Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.