Anti-Cyclophilin A抗体(ab3563)

Abreviews (4)Q&A (7)Specific References (6)

概述

  • 产品名称
    Anti-Cyclophilin A抗体
    参阅全部 Cyclophilin A 一抗
  • 描述
    兔多克隆抗体to Cyclophilin A
  • 宿主
    Rabbit
  • 特异性
    Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Human
    预测可用于: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
  • 免疫原

    Other Immunogen Type corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.

性能

应用

Our Abpromise guarantee covers the use of ab3563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300.
ELISA 1/1000.

Used for 2 hrs on Rabbit liver cells.
ICC Use at an assay dependent concentration.

靶标

图片

  • Western blot - Anti-Cyclophilin A antibody (ab3563)
    Western blot - Anti-Cyclophilin A antibody (ab3563)

    ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
    ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Cyclophilin A antibody (ab3563)
    Western blot - Anti-Cyclophilin A antibody (ab3563)
    All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : NIH-3T3

    Lysates/proteins at 25 µg per lane.

    Observed band size: 18 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Chen J  et al. Human Cytomegalovirus Encoded miR-US25-1-5p Attenuates CD147/EMMPRIN-Mediated Early Antiviral Response. Viruses 9:N/A (2017). Read more (PubMed: 29194430) »
  • Fan YN  et al. Mir-34a Mimics Are Potential Therapeutic Agents for p53-Mutated and Chemo-Resistant Brain Tumour Cells. PLoS One 9:e108514 (2014). WB . Read more (PubMed: 25250818) »

See all 6 Publications for this product

发表研究结果有使用 ab3563？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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11 reviews or Q&A

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Western blot abreview for Anti-Cyclophilin A antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
skin
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Nov 23 2016

Western blot abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MeT5a)
Loading amount
15 µg
Specification
MeT5a
Gel Running Conditions
Reduced Denaturing (10% Acrylamide, Laemmli buffer system)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 09 2008

Our lab is interested in purchasing ab3563. Can you provide us with the concentration of the antibody or the mass contained in one unit of this product?

Thank you for your enquiry. This product ab3563 is supplied as 400 µl of total IgG in PBS containing 0.05% sodium azide. Unfortunately, the concentration of this product has not been determined at this time, as the antibody has only been protein (a...

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What is the concentration of this product?

Thank you for your enquiry. This antibody is listed on the data sheet as being an IgG fraction. Because this antibody is not affinity purified, the concentration is undetermined. I understand that you need the concentration for your assays, but unfortu...

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What is the concentration of ab3563?

Thank you for your enquiry. Regarding polyclonal antibody to Cyclophilin A ab3563, our source has not measured the IgG concentration. The activity of some of the antibodies we sell is based on ELISA data. However,the concentration is expected to be...

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ELISA abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Purified protein (competitive ELISA assay)
Specification
competitive ELISA assay
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Type
Other
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Verified customer

提交于 Jul 31 2006

ELISA abreview for Anti-Cyclophilin A antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rabbit Cell (rabbit liver cells)
Specification
rabbit liver cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2
Type
Other
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Abcam user community

Verified customer

提交于 Jul 28 2006

Hi, do you reckon i could detect the CyPA in my cardia protein extracts to use it as a housekeeping? Thanks

Thank you for your enquiry. Cyclophilin A is described as a housekeeoping gene in the literature. However, this cyclophilin A antibody does not detect endogenous levels of CyPA, so it would not be suitable for this purpose. I hope this informati...

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I just purchased your product rabbit polyclonal to cyclophilin A(ab3563-400, \ Lot # 162382). Could you let me know the protein or IgG concentration of the antibody.

I apologize, but it looks like the IgG concentration is unknown for ab3563 (Cyclophilin A). Occasionally, ELISA tests are used to determine activity instead of measuring the protein concentration. More than likely, the range falls between 0.5-2 mg/ml. ...

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BATCH NUMBER 75863 ORDER NUMBER 2005a285 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Human HeLa cell cell extract PRIMARY ANTIBODY ab3563 anti-Cyclophilin PAb 1:300 dilution for one hour, wash three times with ...

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I'm sorry to hear you are experiencing problems with ab3563. I would like to recommend incubating the membrane in the antibody solution overnight at 4C. Please let me know if you still have problems following this recommendation and I will arrange a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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