兔多克隆抗体to Cyclin T2
Recombinant fragment corresponding to a region within amino acids 497 and 707 of Human Cyclin T2 (NP_490595).
WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates
ICC/IF: Hela cell
IHC-P: H1299 Xenograft
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 81 kDa.
1/100 - 1/250.
1/100 - 1/200.
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
Belongs to the cyclin family. Cyclin C subfamily.
Information by UniProt
Cyclin T2a antibody
Western blot - Anti-Cyclin T2 antibody (ab96133)
Anti-Cyclin T2 antibody (ab96133) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 81 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin T2 antibody (ab96133)
ab96133, at a 1/200 dilution, staining Cyclin T2 in paraformaldehyde fixed A431 cells by Immunofluorescence.
Lower image: merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T2 antibody (ab96133)
ab96133, at a 1/100 dilution, staining Cyclin T2 in paraffin embedded H1299 xenograft by Immunohistochemistry.
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