概述

  • 产品名称Anti-Cyclin T1抗体
    参阅全部 Cyclin T1 一抗
  • 描述
    兔多克隆抗体to Cyclin T1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2 - 51 (EGERKNNNKR WYFTREQLEN SPSRRFGVDP DKELSYRQQA ANLLQDMGQR) of human Cyclin T1 (NP_001231).

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

靶标

  • 功能Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the cyclin family. Cyclin C subfamily.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T1 antibody 图像



  • Predicted band size : 81 kDa
    Western blot analysis of HeLa cell lysate labeling Cyclin T1 with ab83314 at 1.0µg/ml.
  • Anti-Cyclin T1 antibody (ab83314) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,88 kDa. We are unsure as to the identity of these extra bands.

Anti-Cyclin T1 antibody (ab83314)参考文献

ab83314 has not yet been referenced specifically in any publications.

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