概述

  • 产品名称Anti-Cyclin T1抗体
    参阅全部 Cyclin T1 一抗
  • 描述
    羊多克隆抗体to Cyclin T1
  • 经测试应用适用于: WB, IP, EMSAmore details
  • 种属反应性
    与反应: Mouse, Horse, Human, Monkey
    不与反应: Drosophila melanogaster
  • 免疫原

    Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

  • 常规说明


    ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

性能

应用

Our Abpromise guarantee covers the use of ab27963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration. PubMed: 17576689

靶标

  • 功能Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the cyclin family. Cyclin C subfamily.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN T1 antibody
    • CCNT antibody
    • CCNT 1 antibody
    • Ccnt1 antibody
    • CCNT1_HUMAN antibody
    • CDK9 associated C type protein antibody
    • Cyc T1 antibody
    • Cyclin C related protein antibody
    • Cyclin T antibody
    • cyclin T1 antibody
    • Cyclin T1b antibody
    • Cyclin-T antibody
    • Cyclin-T1 antibody
    • CYCT 1 antibody
    • CycT1 antibody
    • HIVE1 antibody
    • Human immunodeficiency virus 1 expression antibody
    • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
    • pTEFb subunit antibody
    • Subunit of positive elongation transcription factor b antibody
    see all

Anti-Cyclin T1 antibody 图像

  • All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa cell nuclear lysate
    Lane 3 : monkey Vero cell lysate
    Lane 4 : mouse kidney cell lysate
    Lane 5 : Drosophila S2 cell lysate
    Lane 6 : horse dermal cell lysate

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 81 kDa
    Observed band size : 87 kDa (why is the actual band size different from the predicted?)

Anti-Cyclin T1 antibody (ab27963)参考文献

This product has been referenced in:
  • Krueger BJ  et al. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Nucleic Acids Res : (2008). WB ; Human . Read more (PubMed: 18281698) »
  • Sedore SC  et al. Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR. Nucleic Acids Res 35:4347-58 (2007). EMSA ; Human . Read more (PubMed: 17576689) »

See all 2 Publications for this product

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