概述

  • 产品名称Anti-Cyclin H抗体[EPR3929]
    参阅全部 Cyclin H 一抗
  • 描述
    兔单克隆抗体[EPR3929] to Cyclin H
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Cyclin H.

  • 阳性对照
    • Jurkat, A431, K562 and HeLa cell lysates, Human breast carcinoma tissue, HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.

靶标

  • 功能Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • 序列相似性Belongs to the cyclin family. Cyclin C subfamily.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6330408H09Rik antibody
    • AI661354 antibody
    • AV102684 antibody
    • AW538719 antibody
    • CAK antibody
    • CAK complex subunit antibody
    • ccnh antibody
    • CCNH_HUMAN antibody
    • CDK activating kinase antibody
    • CDK activating kinase complex subunit antibody
    • Cyclin dependent kinase activating kinase antibody
    • cyclin dependent kinase activating kinase complex subunit antibody
    • Cyclin H antibody
    • Cyclin-H antibody
    • CyclinH antibody
    • MO15 associated protein antibody
    • MO15-associated protein antibody
    • p34 antibody
    • p36 antibody
    • p37 antibody
    see all

Anti-Cyclin H antibody [EPR3929] 图像

  • All lanes : Anti-Cyclin H antibody [EPR3929] (ab92376)

    Lane 1 : Jurkat cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 38 kDa
  • ab92376 staining Cyclin H in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. (Detection: DAB staining)
  • ab92376 staining Cyclin H in HeLa cells by Immunofluorescence.

Anti-Cyclin H antibody [EPR3929] (ab92376)参考文献

This product has been referenced in:
  • Brasier AR  et al. RelA Ser276 phosphorylation-coupled Lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. J Virol 85:11752-69 (2011). WB ; Human . Read more (PubMed: 21900162) »

See 1 Publication for this product

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