概述

性能

应用

Our Abpromise guarantee covers the use of ab5502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21423799
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May play a role in growth regulation and in negative regulation of cell cycle progesssion.
  • 组织特异性High levels in cerebellum, thymus, spleen and prostate. Low levels in skeletal muscle.
  • 序列相似性Belongs to the cyclin family. Cyclin G subfamily.
  • 发展阶段Expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNG 2 antibody
    • Ccng2 antibody
    • CCNG2 protein antibody
    • CCNG2_HUMAN antibody
    • Cyclin-G2 antibody
    • CyclinG2 antibody
    see all

Anti-Cyclin G2 antibody 图像



  • Predicted band size : 39 kDa


    ab5502 at a 1/1000 dilution staining approximately 39 kDa Cyclin G2 in mouse muscle extract by Western blot (ECL).

Anti-Cyclin G2 antibody (ab5502)参考文献

This product has been referenced in:
  • Fujimura A  et al. Expression of a constitutively active calcineurin encoded by an intron-retaining mRNA in follicular keratinocytes. PLoS One 6:e17685 (2011). WB, IHC-Fr ; Human . Read more (PubMed: 21423799) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary)
Specification Mammary
Fixative Paraformaldehyde
Permeabilization Yes - Citrate pH6 antigen retrieval + 0.025% Triton X permeabilization
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 01 2011

I understand your concern regarding the non specific bands in the myoblast cell lysate and the human cancer cell line lysate. Cancer cell lines can undergo different morphological, biochemical and molecular biological changes especially after certain p...

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Thank you for your enquiry and for taking your time to fill in the Questionnaire. We are very sorry to hear that you are having problem with this antibody. 1. Ab5502 has been tested and characterised for Western blot application using mouse muscle ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"