概述

  • 产品名称
    Anti-Cyclin G2抗体
    参阅全部 Cyclin G2 一抗
  • 描述
    小鼠单克隆抗体to Cyclin G2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human, Recombinant fragment
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-345 of Human Cyclin G2

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab54901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      May play a role in growth regulation and in negative regulation of cell cycle progesssion.
    • 组织特异性
      High levels in cerebellum, thymus, spleen and prostate. Low levels in skeletal muscle.
    • 序列相似性
      Belongs to the cyclin family. Cyclin G subfamily.
    • 发展阶段
      Expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCNG 2 antibody
      • Ccng2 antibody
      • CCNG2 protein antibody
      • CCNG2_HUMAN antibody
      • Cyclin-G2 antibody
      • CyclinG2 antibody
      see all

    图片

    • Western blot against tagged recombinant protein immunogen using ab54901 Cyclin G2 antibody at 1ug/ml. Predicted band size of recombinant, tagged immunogen is 64 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • ab54901 at 10 ug/ml staining Cyclin G2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    • All lanes : Anti-Cyclin G2 antibody (ab54901) at 1/1000 dilution (in TBST + 5% milk for 16 hours at 4°C)

      Lane 1 : Whole cell lysate of Human U87MG tumour cell line treated with DMSO
      Lane 2 : Whole cell lysate of Human U87MG tumour cell line treated with 1µM LY294002 for 6 hours
      Lane 3 : Whole cell lysate of Human U87MG tumour cell line treated with 10µM LY294002 for 6 hours

      Lysates/proteins at 50 µg per lane.

      Secondary
      An HRP-conjugated Goat anti-mouse polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 64 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of an anonymous Abreview

      Blocking Step : 5% Milk for 1 hour at 23°C

      See Abreview

    文献

    This product has been referenced in:
    • Hofstetter CP  et al. Protein phosphatase 2A mediates dormancy of glioblastoma multiforme-derived tumor stem-like cells during hypoxia. PLoS One 7:e30059 (2012). WB ; Human . Read more (PubMed: 22253878) »

    See 1 Publication for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87MG tumour cell line)
    Loading amount
    50 µg
    Specification
    U87MG tumour cell line
    Treatment
    0.1 and 10uM LY294002 for 6h
    Gel Running Conditions
    Reduced Denaturing (10% tris glycine)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 09 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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