概述

  • 产品名称Anti-Cyclin E2抗体[EP454Y]
    参阅全部 Cyclin E2 一抗
  • 描述
    兔单克隆抗体[EP454Y] to Cyclin E2
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Jurkat cell lysate, HeLa cells, human breast carcinoma (IHC-P).
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin E2 antibody (Alexa Fluor® 488) [EP454Y] (ab200423)

    Anti-Cyclin E2 antibody (Alexa Fluor® 647) [EP454Y] (ab200424)

    Anti-Cyclin E2 antibody (HRP) [EP454Y] (ab200425)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab40890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
IP 1/50.

靶标

  • 相关性The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Anti-Cyclin E2 antibody [EP454Y] 图像

  • Anti-Cyclin E2 antibody [EP454Y] (ab40890) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Immunofluorescent staining of staining HeLa cells using ab40890 (1/100).
  • ab40890 (1/250) staining human breast carcinoma by immunohistochemistry, paraffin-embedded tissue.

Anti-Cyclin E2 antibody [EP454Y] (ab40890)参考文献

This product has been referenced in:
  • Scharfmann R  et al. Development of a conditionally immortalized human pancreatic ß cell line. J Clin Invest 124:2087-98 (2014). WB ; Human . Read more (PubMed: 24667639) »
  • Lecona E  et al. Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes. PLoS Biol 11:e1001737 (2013). WB, IP ; Human . Read more (PubMed: 24358021) »

See all 9 Publications for this product

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