概述

  • 产品名称Anti-Cyclin E2抗体
    参阅全部 Cyclin E2 一抗
  • 描述
    兔多克隆抗体to Cyclin E2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (TQDVKKRREE VTKKHQYEIR NCWPPVLSGG ISPCIIIETP HKEIGTSDFS) of Human Cyclin E2 (NP_477097).

  • 阳性对照
    • OVCAR-3 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab94724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Anti-Cyclin E2 antibody 图像

  • Anti-Cyclin E2 antibody (ab94724) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR-3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/5000 dilution

    Predicted band size : 47 kDa

Anti-Cyclin E2 antibody (ab94724)参考文献

ab94724 has not yet been referenced specifically in any publications.

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