Anti-Cyclin E2抗体(ab78941)

概述

  • 产品名称
    Anti-Cyclin E2抗体
    参阅全部 Cyclin E2 一抗
  • 描述
    兔多克隆抗体to Cyclin E2
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Cyclin E2 around the phosphorylation site of threonine 392 (I-M-TP-P-P).

  • 阳性对照
    • Human thyroid gland tissue. Extracts from COLO cells and Jurkat cells. NIH/3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab78941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性
    The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • CCN E2 antibody
    • CCNE 2 antibody
    • CCNE2 antibody
    • CCNE2 protein antibody
    • CYC E2 antibody
    • CYCE 2 antibody
    • CYCE2 antibody
    • CyclinE2 antibody
    • G1/S specific cyclin E2 antibody
    see all

Anti-Cyclin E2 antibody 图像

  • All lanes : Anti-Cyclin E2 antibody (ab78941) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using ab78941 at 1/50 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
  • Immunofluorescence analysis of NIH/3T3 cells using ab78941 at 1/500 dilution in the absence (left panel) or presence (right panel) of immunising peptide.

Anti-Cyclin E2 antibody (ab78941)参考文献

ab78941 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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