RabMAb

Anti-Cyclin E1 (phospho T77)抗体[EPR2242(2)] (ab124696)

概述

  • 产品名称Anti-Cyclin E1 (phospho T77)抗体[EPR2242(2)]
    参阅全部 Cyclin E1 一抗
  • 描述
    兔单克隆抗体[EPR2242(2)] to Cyclin E1 (phospho T77)
  • 特异性ab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues surrounding Threonine 77 of Human Cyclin E1.

  • 阳性对照
    • JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

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应用

Our Abpromise guarantee covers the use of ab124696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Essential for the control of the cell cycle at the G1/S (start) transition.
    • 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
    • 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] 图像

    • All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution

      Lane 1 : JAR cell lysate, untreated
      Lane 2 : JAR cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 47 kDa
    • ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696)参考文献

    ab124696 has not yet been referenced specifically in any publications.

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