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RabMAb

Anti-Cyclin E1 (phospho T77)抗体[EPR2242(2)] (ab124696)

概述

  • 产品名称
    Anti-Cyclin E1 (phospho T77)抗体[EPR2242(2)]
    参阅全部 Cyclin E1 一抗
  • 描述
    兔单克隆抗体[EPR2242(2)] to Cyclin E1 (phospho T77)
  • 特异性
    ab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
  • 经测试应用
    适用于: WB, IHC-P, ICCmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues surrounding Threonine 77 of Human Cyclin E1.

  • 阳性对照
    • JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Essential for the control of the cell cycle at the G1/S (start) transition.
    • 组织特异性
      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • 序列相似性
      Belongs to the cyclin family. Cyclin E subfamily.
    • 翻译后修饰
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    图片

    • All lanes : Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] (ab124696) at 1/1000 dilution

      Lane 1 : JAR cell lysate, untreated
      Lane 2 : JAR cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 47 kDa
    • ab124696, at 1/100, staining Cyclin E1 (phospho T77) in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab124696 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Nov 06 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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