Anti-Cyclin E1 (phospho T395)抗体(ab52195)

概述

  • 产品名称Anti-Cyclin E1 (phospho T395)抗体
    参阅全部 Cyclin E1 一抗
  • 描述
    兔多克隆抗体to Cyclin E1 (phospho T395)
  • 特异性This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1 (phospho T395). Immunogen is in the range of aa 361-410
    Database link: P24864

  • 阳性对照
    • Human colon carcinoma tissue and extracts from Hela cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 (phospho T395) antibody 图像

  • All lanes : Anti-Cyclin E1 (phospho T395) antibody (ab52195) at 1/500 dilution

    Lane 1 : HeLa cells treated with Paclitaxel 1uM 60 minutes with phospho peptide
    Lane 2 : HeLa cells treated with Paclitaxel 1uM 60 minutes with phospho peptide


    Predicted band size : 50 kDa
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ab52195 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ab52195 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

  • Ab52195 staining human normal placenta. Staining is localized to the nucleus.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab52195 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cyclin E1 (phospho T395) antibody (ab52195)参考文献

ab52195 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This antibody is specific for human cyclin E1, but not for E2. The partial sequence used for immunogen is SGLLT(p)PP. The whole protein sequence of E1 is located at Swiss prot website of http://www.expasy.org/uniprot/P24864....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"