Anti-Cyclin E1 (phospho T395)抗体(ab52195)
Key features and details
- Rabbit polyclonal to Cyclin E1 (phospho T395)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Cyclin E1 (phospho T395)抗体
参阅全部 Cyclin E1 一抗 -
描述
兔多克隆抗体to Cyclin E1 (phospho T395) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human Cyclin E1 (phospho T395). Immunogen is in the range of aa 361-410
Database link: P24864 -
阳性对照
- Human colon carcinoma tissue and extracts from Hela cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52195于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
-
功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
组织特异性
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
序列相似性
Belongs to the cyclin family. Cyclin E subfamily. -
翻译后修饰
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 898 Human
- Entrez Gene: 12447 Mouse
- Omim: 123837 Human
- SwissProt: P24864 Human
- SwissProt: Q61457 Mouse
- Unigene: 244723 Human
- Unigene: 16110 Mouse
-
别名
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
图片
-
All lanes : Anti-Cyclin E1 (phospho T395) antibody (ab52195) at 1/500 dilution
All lanes : HeLa cells treated with Paclitaxel 1uM 60 minutes with phospho peptide
Predicted band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ab52195 at a 1/50 dilution.
Left image un-treated.
Right image treated with phosphopeptide.
-
Ab52195 staining human normal placenta. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
ICC/IF image of ab52195 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52195, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab52195 尚未被引用在任何文献中。