概述

  • 产品名称Anti-Cyclin E1抗体
    参阅全部 Cyclin E1 一抗
  • 描述
    小鼠多克隆抗体to Cyclin E1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to Human Cyclin E1.

  • 阳性对照
    • Human colon tissue lysate, Cyclin E1 transfected 293T cell line lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab89585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody 图像

  • Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • All lanes : Anti-Cyclin E1 antibody (ab89585) at 1 µg/ml

    Lane 1 : Cyclin E transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • ab89585 at 10 µg/ml staining Cyclin E in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

Anti-Cyclin E1 antibody (ab89585)参考文献

ab89585 has not yet been referenced specifically in any publications.

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