Anti-Cyclin E1抗体(ab74276)

概述

  • 产品名称
    Anti-Cyclin E1抗体
    参阅全部 Cyclin E1 一抗
  • 描述
    兔多克隆抗体to Cyclin E1
  • 经测试应用
    适用于: Functional Studies, ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide from the C-terminal of human cyclin E.

  • 阳性对照
    • SKBR3 and MAD109 cells. Breast carcinoma or placenta.

性能

应用

Our Abpromise guarantee covers the use of ab74276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies
ICC/IF
IP
WB
IHC-P
  • 应用说明
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
    Kinase Assay: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Essential for the control of the cell cycle at the G1/S (start) transition.
    • 组织特异性
      Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • 序列相似性
      Belongs to the cyclin family. Cyclin E subfamily.
    • 翻译后修饰
      Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    图片

    • ab74276, at 1/50 dilution, staining Cyclin E in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

    文献

    ab74276 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Hela
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Nov 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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