Anti-Cyclin E1抗体(ab37541)

概述

  • 产品名称Anti-Cyclin E1抗体
    参阅全部 Cyclin E1 一抗
  • 描述
    兔多克隆抗体to Cyclin E1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab38085, ab38084, ab38084)

  • 阳性对照
    • ab37541 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line) U2OS (Human osteosarcoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab37541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody 图像

  • All lanes : Anti-Cyclin E1 antibody (ab37541) at 1/250 dilution

    Lane 1 : A431 whole cell lysate (ab7909)
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,41 kDa. We are unsure as to the identity of these extra bands.

Anti-Cyclin E1 antibody (ab37541)参考文献

ab37541 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Jun 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"