- 产品名称Anti-Cyclin E1抗体
参阅全部 Cyclin E1 一抗
兔多克隆抗体to Cyclin E1
Synthetic peptide corresponding to Human Cyclin E1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
(Peptide available as
ab38085, ab38084, ab38084)
- ab37541 gives a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK 293 (Human embryonic kidney cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)
MCF-7 (Human breast adenocarcinoma cell line)
U2OS (Human osteosarcoma cell line)
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 47 kDa).
- 功能Essential for the control of the cell cycle at the G1/S (start) transition.
- 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
- 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
- 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
Anti-Cyclin E1 antibody 图像
Western blot - Anti-Cyclin E1 antibody (ab37541)
All lanes : Anti-Cyclin E1 antibody (ab37541) at 1/250 dilution
Lane 1 : A431 whole cell lysate (ab7909)
Lane 2 : Jurkat whole cell lysate (ab7899)
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa,41 kDa. We are unsure as to the identity of these extra bands.
Anti-Cyclin E1 antibody (ab37541)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"