概述

  • 产品名称Anti-Cyclin E1抗体
    参阅全部 Cyclin E1 一抗
  • 描述
    兔多克隆抗体to Cyclin E1
  • 特异性Antiserum will specifically react with a 45 kDa Cyclin E1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin E1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Immunogen: Repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E1.
    Database link: P24864

性能

应用

Our Abpromise guarantee covers the use of ab2094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP 1/100.
ELISA 1/100000.

靶标

  • 功能Essential for the control of the cell cycle at the G1/S (start) transition.
  • 组织特异性Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
  • 序列相似性Belongs to the cyclin family. Cyclin E subfamily.
  • 翻译后修饰Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNE antibody
    • Ccne1 antibody
    • CCNE1_HUMAN antibody
    • cyclin E variant ex5del antibody
    • cyclin E variant ex7del antibody
    • Cyclin E1 antibody
    • Cyclin Es antibody
    • Cyclin Et antibody
    • CyclinE antibody
    • G1/S specific cyclin E antibody
    • G1/S-specific cyclin-E1 antibody
    see all

Anti-Cyclin E1 antibody (ab2094)参考文献

This product has been referenced in:
  • Ferguson RL & Maller JL Cyclin E-dependent localization of MCM5 regulates centrosome duplication. J Cell Sci 121:3224-32 (2008). ICC/IF ; Human . Read more (PubMed: 18799789) »
  • Rao HV  et al. Cyclin D1, cdk4, and Bim are involved in thrombin-induced apoptosis in cultured cortical neurons. J Neurochem 101:498-505 (2007). WB ; Rat . Read more (PubMed: 17254021) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The immunogen for this antibody is a full length synthetic polypeptide corresponding to the human gene sequence. I have updated the datasheet to reflect this. I hope this information helps, please do not hesitate to c...

Read More

This antibody has not yet been tested on immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"