Anti-Cyclin D3抗体(ab63535)

概述

  • 产品名称Anti-Cyclin D3抗体
    参阅全部 Cyclin D3 一抗
  • 描述
    兔多克隆抗体to Cyclin D3
  • 特异性This antibody detects endogenous levels of total Cyclin D3 protein.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized non-phosphopeptide derived from human Cyclin D3 around the phosphorylation site of threonine 283 (T-S-TP-P-T).

  • 阳性对照
    • COS7 cell lysate or human lung carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab63535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • 序列相似性Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • 细胞定位Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Anti-Cyclin D3 antibody 图像

  • All lanes : Anti-Cyclin D3 antibody (ab63535) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells (5-30ug), treated with Forskolin (40nM, 30mins), without immunising peptide.
    Lane 2 : Extracts from COS7 cells (5-30ug), treated with Forskolin (40nM, 30mins), with immunising peptide (5-10ug).


    Predicted band size : 35 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Ab63535 (1/50-1/100) staining human Cyclin D3 in human lung carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.

Anti-Cyclin D3 antibody (ab63535)参考文献

ab63535 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse retinal cells)
Loading amount 40 µg
Specification mouse retinal cells
Gel Running Conditions Reduced Denaturing (4-12% gradient gel, sample was denatured at 70C for 10mins)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 29 2011

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