Anti-Cyclin D3/CCND3抗体(ab112034)
Key features and details
- Rabbit polyclonal to Cyclin D3/CCND3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin D3/CCND3抗体
参阅全部 Cyclin D3/CCND3 一抗 -
描述
兔多克隆抗体to Cyclin D3/CCND3 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 69% homology with Cyclin D2. Cross-reactivity with this protein has not been confirmed experimentally. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Baboon, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Saos-2 whole cell lysates. ICC/IF: formaldehyde fixed WERI cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Abcam recommends using milk as the blocking agent (1%)
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Abcam recommends using milk as the blocking agent (1%) |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
细胞定位
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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数据库链接
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 100009038 Rabbit
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
see all -
别名
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
图片
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ab112034 staining Cyclin D3/CCND3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab112034 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/ml (Milk Blocking - 1%) + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab112034 被引用在 7 文献中.
- Meng F et al. Knockdown of TRIM44 inhibits the progression of ovarian cancer and is related to the FOXM1-EZH2 signaling pathway. Transl Cancer Res 11:414-425 (2022). PubMed: 35281418
- Han Z et al. DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway. Front Oncol 12:1036401 (2022). PubMed: 36408174
- Xu Y et al. Inhibition of miRNA-152-3p enhances diabetic wound repair via upregulation of PTEN. Aging (Albany NY) 12:14978-14989 (2020). PubMed: 32620711
- Xu Y et al. Inhibition of exosomal miR-24-3p in diabetes restores angiogenesis and facilitates wound repair via targeting PIK3R3. J Cell Mol Med 24:13789-13803 (2020). PubMed: 33142041
- Wang J et al. Protein inhibitor of activated STAT xa depresses cyclin D and cyclin D kinase, and contributes to the inhibition of osteosarcoma cell progression. Mol Med Rep 13:1645-52 (2016). PubMed: 26708148
- Yang P et al. Inhibition of osteosarcoma cell progression by MacroH2A via the downregulation of cyclin D and cyclin-dependent kinase genes. Mol Med Rep 11:1905-10 (2015). PubMed: 25378143
- Lei S et al. MacroH2A suppresses the proliferation of the B16 melanoma cell line. Mol Med Rep 10:1845-50 (2014). PubMed: 25119498