Anti-Cyclin C (phospho S275)抗体(ab78868)

概述

  • 产品名称Anti-Cyclin C (phospho S275)抗体
    参阅全部 Cyclin C 一抗
  • 描述
    兔多克隆抗体to Cyclin C (phospho S275)
  • 特异性Detects endogenous levels of Cyclin C only when phosphorylated at serine 275 (Human: Ser275; Mouse: Ser275; Rat: Ser270).
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Cyclin C around the phosphorylation site of serine 275 (N-G-SP-Q-N)

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue. WB: extracts from UV-treated 3T3 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab78868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
  • 组织特异性Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.
  • 序列相似性Belongs to the cyclin family. Cyclin C subfamily.
    Contains 1 cyclin N-terminal domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ccnc antibody
    • CCNC_HUMAN antibody
    • CycC antibody
    • Cyclin C antibody
    • Cyclin-C antibody
    • hSRB11 antibody
    • OTTHUMP00000016897 antibody
    • SRB11 homolog antibody
    see all

Anti-Cyclin C (phospho S275) antibody 图像

  • Left panel: immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using 1/50 ab78868; Right panel: same, after pre-incubation with the immunizing phosphopeptide.
  • All lanes : Anti-Cyclin C (phospho S275) antibody (ab78868) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells treated with UV (15mins)
    Lane 2 : Extracts from 3T3 cells treated with UV (15mins) with immunizing phosphopeptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

Anti-Cyclin C (phospho S275) antibody (ab78868)参考文献

ab78868 has not yet been referenced specifically in any publications.

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