概述

  • 产品名称Anti-Cyclin B3抗体
    参阅全部 Cyclin B3 一抗
  • 描述
    兔多克隆抗体to Cyclin B3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    a region within synthetic peptide: MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP, corresponding to N terminal amino acids 1-50 of Human Cyclin B3

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab66759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 158 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性Cyclin B3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.This cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • CCNB 3 antibody
    • CYCB3 antibody
    • G2/mitotic specific cyclin B3 antibody

Anti-Cyclin B3 antibody 图像

  • Anti-Cyclin B3 antibody (ab66759) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 158 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)

Anti-Cyclin B3 antibody (ab66759)参考文献

ab66759 has not yet been referenced specifically in any publications.

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