概述

  • 产品名称
    Anti-Cyclin B2抗体[X121.10]
    参阅全部 Cyclin B2 一抗
  • 描述
    小鼠单克隆抗体[X121.10] to Cyclin B2
  • 特异性
    ab10839 blots only cyclin B2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Xenopus laevis
  • 免疫原

    Full length native protein (purified) (Xenopus laevis).

  • 表位
    The epitope for X121 lies within a region spanning the amino acids 1-112.
  • 阳性对照
    • Xenopus egg extract.

性能

应用

Our Abpromise guarantee covers the use of ab10839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 序列相似性
    Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Information by UniProt
  • 数据库链接
    • 别名
      • ccnb2 antibody
      • CCNB2_HUMAN antibody
      • CycB2 antibody
      • Cyclin B2 antibody
      • G2 mitotic specific cyclin B2 antibody
      • G2/mitotic specific cyclin B2 antibody
      • G2/mitotic-specific cyclin-B2 antibody
      • HsT17299 antibody
      • MGC108931 antibody
      • MGC140694 antibody
      see all

    图片

    • All lanes : Anti-Cyclin B2 antibody [X121.10] (ab10839) at 1 µg/ml

      Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
      Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)


      Predicted band size : 48 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa (possible cross reactivity),40 kDa (possible cross reactivity).

    文献

    This product has been referenced in:
    • Moore JD  et al. Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 13:4388-400 (2002). Read more (PubMed: 12475960) »
    • Bodart JF  et al. Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-21 (1999). Read more (PubMed: 10585264) »

    See all 2 Publications for this product

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