概述

  • 产品名称Anti-Cyclin B1 (phospho S147)抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1 (phospho S147)
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S147). Immunogen range is aa 121-170.
    Database link: P14635

  • 阳性对照
    • Human placenta tissue, Jurkat cell extracts treated with UV light.

性能

应用

Our Abpromise guarantee covers the use of ab60986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).

靶标

Anti-Cyclin B1 (phospho S147) antibody 图像

  • All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
    Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : >48 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.

Anti-Cyclin B1 (phospho S147) antibody (ab60986)参考文献

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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