概述

  • 产品名称Anti-Cyclin B1 (phospho S126)抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1 (phospho S126)
  • 特异性ab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
    Database link: P14635

  • 阳性对照
    • human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

性能

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应用

Our Abpromise guarantee covers the use of ab55184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19376971
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

靶标

Anti-Cyclin B1 (phospho S126) antibody 图像

  • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
    Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.

Anti-Cyclin B1 (phospho S126) antibody (ab55184)参考文献

This product has been referenced in:
  • Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »
  • Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse . Read more (PubMed: 19376971) »

See all 3 Publications for this product

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