Anti-Cyclin B1 (phospho S126)抗体(ab3488)

概述

  • 产品名称Anti-Cyclin B1 (phospho S126)抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1 (phospho S126)
  • 特异性Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Hamster, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
    Sequence: ILVDTASpPSPME
    Database link: P14635

  • 常规说明


    This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

性能

应用

Our Abpromise guarantee covers the use of ab3488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

靶标

Anti-Cyclin B1 (phospho S126) antibody 图像

  • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : H23 whole cell lysate
    Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
    Lane 4 : Glioblastoma (T98G) cell lysate
    Lane 5 : B cell lymphoblast (Daudi) cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa


    Exposure time : 20 seconds

Anti-Cyclin B1 (phospho S126) antibody (ab3488)参考文献

ab3488 has not yet been referenced specifically in any publications.

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