RabMAb

Anti-Cyclin B1抗体[Y106] (ab32053)

概述

  • 产品名称Anti-Cyclin B1抗体[Y106]
    参阅全部 Cyclin B1 一抗
  • 描述
    兔单克隆抗体[Y106] to Cyclin B1
  • 特异性This antibody is specific for Human cyclin B1. It does not cross-react with other cyclin family members.
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1.

  • 表位ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
  • 阳性对照
    • Jurkat and Hela cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this product.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Cyclin B1 antibody (HRP) [Y106] (ab193977)

    Anti-Cyclin B1 antibody (Phycoerythrin) [Y106] (ab210615)

性能

应用

Our Abpromise guarantee covers the use of ab32053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000 - 1/20000. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/50.
IP 1/100.

靶标

  • 功能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻译后修饰Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody [Y106] 图像

  • All lanes : Anti-Cyclin B1 antibody [Y106] (ab32053) at 1/20000 dilution

    Lane 1 : Hela cell lysate.
    Lane 2 : Jurkat cell lysate.


    Predicted band size : 58 kDa
  • ab32053 at a 1:100 dilution staining Human cyclin B1 in human skin carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing Jurkat cells stained with ab32053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32053, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cyclin B1 antibody [Y106] (ab32053)参考文献

This product has been referenced in:
  • Yang T  et al. Anti-tumor Efficiency of Lipid-coated Cisplatin Nanoparticles Co-loaded with MicroRNA-375. Theranostics 6:142-54 (2016). WB ; Human . Read more (PubMed: 26722380) »
  • Gao K  et al. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Biochem Biophys Res Commun 458:849-55 (2015). WB ; Human . Read more (PubMed: 25689719) »

See all 16 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH 8.6, 20 min 100C
Sample Human Tissue sections (tonsil)
Specification tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (MCF7 cells)
Specification MCF7 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 11 2013

Thank you for contacting us.

I can confirm that the abs have IgG1 isotype.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment double thymidine synchronization
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"