概述

  • 产品名称Anti-Cyclin B1抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1
  • 特异性Reacts with cyclin B1
  • 经测试应用适用于: IHC-P, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1 aa 414-433 (C terminal).
    Sequence:

    TLPQLNSALVQDLAKAVAKV


    Database link: P14635
    (Peptide available as ab8012)

  • 阳性对照
    • HeLa and K-562 IHC-P: FFPE human cervix tissue sections.

性能

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应用

Our Abpromise guarantee covers the use of ab7957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • 序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 翻译后修饰Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody 图像



  • Predicted band size : 49 kDa
    ab7957 staining Cyclin B1 in HeLa whole cell lysate ab7957 staining Cyclin B1 in HeLa whole cell lysate
  • IHC image of Cyclin B1 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7957, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cyclin B1 antibody (ab7957)参考文献

This product has been referenced in:
  • Hu P  et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB ; Human . Read more (PubMed: 25370920) »
  • Johansson P  et al. SCF-FBXO31 E3 Ligase Targets DNA Replication Factor Cdt1 for Proteolysis in the G2 Phase of Cell Cycle to Prevent Re-replication. J Biol Chem 289:18514-25 (2014). WB ; Human . Read more (PubMed: 24828503) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 100000 cells
Specification HeLa cells
Treatment Released from a double thymidine block
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step 2% milk and 0.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Username

Dr. Kum Kum Khanna

Verified customer

提交于 Sep 07 2009

ab7957 is made with a peptide mapping to amino acids 414-433 of human origin (this is the carboxy terminus).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"