概述

  • 产品名称Anti-Cyclin B1抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1
  • 特异性Detects endogenous levels of total Cyclin B1 protein.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Cyclin B1. Immunogen range is aa 91-140. Synthetic non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126
    Database link: P14635

  • 阳性对照
    • Extracts from HeLa cells treated with serum.

性能

应用

Our Abpromise guarantee covers the use of ab52187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 2 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-Cyclin B1 antibody 图像

  • All lanes : Anti-Cyclin B1 antibody (ab52187) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with serum (10%, 15min)
    Lane 2 : extracts from HeLa cells, treated with serum (10%, 15min) with blocking peptide


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab52187 (2µg/ml) staining Cyclin B1 in human placenta.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Cyclin B1 antibody (ab52187)参考文献

This product has been referenced in:
  • Park JE  et al. Direct quantification of polo-like kinase 1 activity in cells and tissues using a highly sensitive and specific ELISA assay. Proc Natl Acad Sci U S A 106:1725-1730 (2009). WB ; Mouse . Read more (PubMed: 19181852) »

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