概述

  • 产品名称
    Anti-Cyclin B1抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1
  • 经测试应用
    适用于: ICC/IF, Sandwich ELISA, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Cyclin B1 (C terminal).
    Database link: P14635

  • 阳性对照
    • Jurkat, Ls174T or HeLa cells. Breast carcinoma tissue section.

性能

应用

Our Abpromise guarantee covers the use of ab2949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.2 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.2 µg/ml with Mouse monoclonal [7A9] to Cyclin B1 (ab49215) as Capture.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

靶标

图片

  • Standard Curve for Cyclin B1 (Analyte: Cyclin B1 protein (His tag) (ab40591)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [7A9] to Cyclin B1 (ab49215) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to Cyclin B1 (ab2949) at 0.2µg/ml.
  • Breast carcinoma stained with ab2949
  • ab2949 staining Cyclin B1 in mouse MEF cells by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde and blocking with 2% serum was performed for 2 hours at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.3% Triton X-100 in PBS) for 18 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to rabbit IgG was used diluted 1/1000 as secondary antibody. Red is cyclinB1 staining, blue is DAPI staining DNA for nuclei, green is vinculin staining.

    See Abreview

文献

This product has been referenced in:
  • Kauerova T  et al. Antiproliferative and Pro-Apoptotic Effect of Novel Nitro-Substituted Hydroxynaphthanilides on Human Cancer Cell Lines. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27483236) »
  • Metchat A  et al. An actin-dependent spindle position checkpoint ensures the asymmetric division in mouse oocytes. Nat Commun 6:7784 (2015). IF . Read more (PubMed: 26174204) »

See all 6 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Cell lysate - whole cell (Epithelial OVCAR c)
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Loading amount
100 µg
Treatment
Perifosine 0, 12, 24 hr
Specification
Human Cell lysate - whole cell (Epithelial OVCAR c
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2017

Thank you for contacting us.

Unfortunately the immunogen sequence is confidential. It is located within aa 370-433 of P14635 (Uniprot).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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Thank you for confirming that.

I have generated the testing discount code as follows:

Discount code: xxxxxx

Expiration date: 5th December 2012

The terms and conditions of the testing discount scheme can be found f...

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Thank you for contacting us and sorry for the delay in getting back to you.

As requested, I have looked into the sequence homology between the immunogen used to raise antibodies mentioned and the relevant canineprotein sequence. I have to men...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2009

Thank you for your e-mail and forwarding more information about your Western blot assay. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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