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Our Abpromise guarantee covers the use of ab2949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.2 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.2 µg/ml with Mouse monoclonal [7A9] to Cyclin B1 (ab49215) as Capture.|
|IHC-P||Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at 10 µg/mg of lysate.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).|
ab2949 staining Cyclin B1 in mouse MEF cells by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde and blocking with 2% serum was performed for 2 hours at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.3% Triton X-100 in PBS) for 18 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to rabbit IgG was used diluted 1/1000 as secondary antibody. Red is cyclinB1 staining, blue is DAPI staining DNA for nuclei, green is vinculin staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"