概述

  • 产品名称Anti-Cyclin B1抗体
    参阅全部 Cyclin B1 一抗
  • 描述
    兔多克隆抗体to Cyclin B1
  • 特异性Antiserum will specifically react with a 62 kDa Cyclin B1 protein. No reaction was observed against other related cyclins.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein corresponding to Cyclin B1 aa 1-433. full length fusion protein
    Database link: P14635

性能

应用

Our Abpromise guarantee covers the use of ab2096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent dilution.
ELISA 1/500 - 1/2000.

靶标

Anti-Cyclin B1 antibody (ab2096)参考文献

This product has been referenced in:
  • Kotecha M  et al. Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78. Mol Cancer Ther 7:1319-28 (2008). WB ; Mouse . Read more (PubMed: 18483319) »
  • Morris C & Jalinot P Silencing of human Int-6 impairs mitosis progression and inhibits cyclin B-Cdk1 activation. Oncogene 24:1203-11 (2005). Read more (PubMed: 15558017) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (embryos before MBT)
Loading amount 1 cells
Specification embryos before MBT
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jolanta Kisielewska

Verified customer

提交于 Apr 16 2010

Antibodies ab2096 (anti-cyclin B1) and ab2094 (anti-cyclin E) have not been tested in immunofluorescence. Ab72 (monoclonal to cyclin B1, V152) can be used for histology.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"