Anti-Cyclin A2抗体[E67.1] (ab39)


  • 产品名称Anti-Cyclin A2抗体[E67.1]
    参阅全部 Cyclin A2 一抗
  • 描述
    小鼠单克隆抗体[E67.1] to Cyclin A2
  • 经测试应用适用于: ICC/IF, WB, ICC, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Cow, Human, African green monkey
  • 免疫原

    Full length protein (Cow).

  • 表位The epitope for this antibody was mapped by phage selection - selected phage contained the sequence LDYP. This would place the epitope within the region spanning amino acids 144 - 148 of human Cyclin A2. Cyclin As that contain the epitope Cow A2 Human A2 Hamster A2 Mouse A2 Cyclin As that do not contain the epitope Chicken A2 Frog A2 Human A1 Mouse A1 Frog A1
  • 常规说明Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.



Our Abpromise guarantee covers the use of ab39 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18077453
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
ICC Use at an assay dependent concentration.
IP Use a concentration of 2.5 µg/ml.
IHC-P Use at an assay dependent concentration.


Anti-Cyclin A2 antibody [E67.1] 图像

  • Cells at stages in the cell cycle from quiescence to mitosis (1-8) were loaded onto SDSPAGE and blotted with anti CDK2 antibody. Note all cells have similar amounts of CDK2. In the lower panel these same extracts were first immunoprecipitated with anti Cyclin A monoclonal E67. In these lanes, only the CDK2 that is complexed to Cyclin A is immunoblotted. Note only the CDK2 in the later stages of the cell cycle is immunoprecipitated, which is when Cyclin A is made, This clearly shows that this antibody is able to immunoprecipitate cyclin A when it is bound to CDK2.
  • ab39 at a 1/100 dilution staining Cyclin A in African Green Monkey COS-7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol and an Alexa-Fluor 555 conjugated anti-mouse secondary was used at a 1/3000 dilution.

Anti-Cyclin A2 antibody [E67.1] (ab39)参考文献

This product has been referenced in:
  • Ge XQ & Blow JJ Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol 191:1285-97 (2010). IP ; Human . Read more (PubMed: 21173116) »
  • Ho KK  et al. Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network. J Biol Chem 283:5477-85 (2008). ICC/IF ; Mouse . Read more (PubMed: 18077453) »

See all 2 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf.

Meine Kollegin von derBestellabteilunghat Ihren Fall an mich weitergeleitet.

Wir haben Ihre Bestellung mit dem abgelaufenen Discountcode für ab39 durchgehen lassen, ich moechte Sie aber bitten, i...

Read More

Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 92.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpalette Teströhrchen zu lagern.
Da nach Ihrer Aussage di...

Read More

Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
Wir verwenden ...

Read More

Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (CV-1)
Total protein in input 500 µg
Specification CV-1
Immuno-precipitation step Protein A/G

Abcam user community

Verified customer

提交于 May 21 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab39 und ab7956 in der grünen Meerkatze zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Si...

Read More

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde bisher noch keiner unsererAnti-Cyclin A Produktein der Westliche Grünmeerkatze (Chlorocebus sabaeus) getestet. Eine BLAST Analy...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample African Green Monkey Cultured Cells (COS-7)
Specification COS-7
Fixative Methanol
Permeabilization Yes - Methanol

Abcam user community

Verified customer

提交于 Mar 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Fibroblast)
Specification Fibroblast
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 for 10mins
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 14 2008

I'm very sorry to hear you are having problems with ab38. We do not have a recommended starting dilution so I would like to recommend trying a higher concentration of antibody (try 10ug/ml). We have only received one complaint regarding this popula...

Read More

1-10 of 15 Abreviews or Q&A