概述

  • 产品名称Anti-Cyclin A2抗体[E399]
    参阅全部 Cyclin A2 一抗
  • 描述
    兔单克隆抗体[E399] to Cyclin A2
  • 特异性ab32498 recognises Cyclin A2.
  • 经测试应用适用于: WB, ICC/IF, IPmore details
    不适用于: Flow Cyt or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin A2 aa 100-200 (N terminal).

  • 表位ab32498 reacts with an epitope located in the N terminal region of Cyclin A2
  • 阳性对照
    • HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250 - 1/500.
IP 1/60.
  • 应用说明Is unsuitable for Flow Cyt or IHC.
  • 靶标

    • 功能Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
    • 序列相似性Belongs to the cyclin family. Cyclin AB subfamily.
    • 发展阶段Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • 细胞定位Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCN1 antibody
      • CCNA antibody
      • Ccna2 antibody
      • CCNA2_HUMAN antibody
      • Cyclin A2 antibody
      • Cyclin-A antibody
      • Cyclin-A2 antibody
      see all

    Anti-Cyclin A2 antibody [E399] 图像

    • Anti-Cyclin A2 antibody [E399] (ab32498) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 48 kDa
      Observed band size : 48 kDa
    • Immunofluorescent analysis of cyclin A expression in HeLa cells using 1/250 ab32498.

    Anti-Cyclin A2 antibody [E399] (ab32498)参考文献

    This product has been referenced in:
    • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . Read more (PubMed: 24846301) »
    • Yajima H  et al. The complexity of DNA double strand breaks is a critical factor enhancing end-resection. DNA Repair (Amst) 12:936-46 (2013). Read more (PubMed: 24041488) »

    See all 3 Publications for this product

    Product Wall

    Ich denke wirklich, dass Ihr Problem mit diesen Aks nicht auf den WB begrenzt ist , da mir die ICC Färbung nicht spezifisch erscheint. Ich würde die Färbung definitiv eher nuklear lokalisiert erwarten, und das ist sie einfach nicht.
    Wir verwenden ...

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    Ich habe Ihnen einen Blot mit dem ab32498 an diese Email gehängt: von einem Schmier keine Spur! Was mir allerdings aufgefallen ist, ist dass der Verdünnungsfaktor um 10 verkleinert wurde. Ich habe unser Datenblatt dementsprechend verändert. Falls ...

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    Thank you for your inquiry. The lab has not tested this antibody for its cross-reactivity with Cyclin A1. But when we BLASTed the immunogen sequence against Cyclin A1, we did not see any significant sequence similarity. Thus, we do not predict ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"