Anti-Cyclin A2抗体- C-terminal (ab227277)
Key features and details
- Rabbit polyclonal to Cyclin A2 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cyclin A2抗体- C-terminal
参阅全部 Cyclin A2 一抗 -
描述
兔多克隆抗体to Cyclin A2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Cow, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human Cyclin A2 (C terminal). The exact sequence is proprietary.
Database link: P20248 -
阳性对照
- WB: Mouse brain tissue lysate. IHC-P: Human lung adenocarcinoma tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. -
序列相似性
Belongs to the cyclin family. Cyclin AB subfamily. -
发展阶段
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
细胞定位
Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER. - Information by UniProt
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数据库链接
- Entrez Gene: 281667 Cow
- Entrez Gene: 890 Human
- Entrez Gene: 12428 Mouse
- Entrez Gene: 100415929 Pig
- Entrez Gene: 114494 Rat
- Omim: 123835 Human
- SwissProt: P30274 Cow
- SwissProt: P20248 Human
see all -
别名
- CCN1 antibody
- CCNA antibody
- Ccna2 antibody
see all
图片
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Anti-Cyclin A2 antibody - C-terminal (ab227277) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa10% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cyclin A2 (green) using ab227277 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: Phalloidin cytoskeletal marker.
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Paraffin-embedded human lung adenocarcinoma tissue stained for Cyclin A2 with ab227277 at 1/250 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227277 尚未被引用在任何文献中。